This monochromator-based instrument allows users to select optimal wavelengths for any chosen fluorophore without the need to purchase additional filter sets. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Table of Contents. FlexStation™ III. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5eThe PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. The cells were incubated with compounds at the indicated doses for 1 h. The day before assay, assay buffer was prepared as described in the product technical manual. on Molecular Devices microplate readers: SpectraMax® iD5, i3x, Paradigm®, and M5 Multi-Mode Microplate Readers. Absorbance was read on a Spectramax M5 Microplate reader at 450 nm. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. SpectraMax® iD5. Detection of pro-inflammatory factorsThe SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Microplate formats. • Tel. SpectraMax® M5 Multi-Mode Microplate Reader MLDVM5 SpectraMax® M5e Multi-Mode Microplate Reader (HTRF certified) MLDVM5E. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. Step 3: Use the Template Editor in the cell standards section of the protocol to create a template of the assay indicating the location of standards (cell numbers for each dilution. Setup for GeneBLAzer® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. In these readers, RLUs are proportional to counts/sec which doesn't change with integration time assuming the signal is constant. If you do not have an iLab account you must first make one: would like to show you a description here but the site won’t allow us. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Note: In this user guide, all references to SpectraMax Multi-Mode. 14 Average %CV 0. Devices’ StakMax® Microplate Stacker in a matter of minutes and . SpectraMax M5 Multi-Mode Microplate Reader. E Models. The dual monochromator optics allow the. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. 4. Fill a clean plate with water. Filterless readers provide consistent results with steady temperature regulation. spectramax m5 multi. PMT sensitivity was set to AutoRange with a target calibration wavelength of 470 nm, and the plate was dark adapted for 1 minute prior to reading. upgradeable with a wide range of high performance capabilities ideal for life science research and drug. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 6: bioNova científica, s. The SpectraMax iD5 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. Thank you!. Fluorescence Microplate Readers. B: Selection of Excitation Wavelength. 95. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. ATP standard curves read on SpectraMax microplate readers. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Lift up the lid over the chamber to insert or remove a cuvette. 0 out of 5 $ 3,799. Offer Ends In: 44 Days 10: 22: 56. Ltd. As readout, fluorescence was measured at Ex/Em = 540/590 nm using a SpectraMax M5 microplate reader with SoftMax Pro v7 (Molecular devices) after 4–6 h of incubation in normal cell-culture medium. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. Supplier: Molecular Devices. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). The assay window spanned four orders of magnitude. Molecular Devices. ) injection of 3 mLThe fluorescence intensity was detected by using a SpectraMax M5 microplate reader from Molecular Devices Co. AlphaScreen. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. 26 1. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). The PBS solution (10. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. Temperature variation ± 0. SpectraMax® M5. SpectraMax iD3, iD5, i3x, i3, M3, M4, M5, M5e, SpectraMax L, 6186 Luminescence FlexStation 3. Expanded wavelength coverage. com 13. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The following document is intended to demonstrate setup of this instrument. p. Microplate Capacity. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Molecular Devices. 7. Readings were taken using a UV–Vis Spectramax M5 ® microplate reader (Molecular Device, USA). The cuvette must be in place when you read the plate. 0 and 0. Sale price $16,495. 3. Small, unbound molecules rotate rapidly in. 72 MB. Fluorescence Microplate Readers. Page 28 Chapter 5: Read Modes and Read Types 1. 7 cells and HEK293T cells were seeded at the density of 2 × 105 cells per well in a 24-well plate overnight. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. Microplate Reader Documentation Version No. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. " The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The dual monochromator optics allow the widest range of applications to be. SpectraMax® M5/M5e Multimode Plate Reader, Molecular Devices. SpectraMax M3, M4, M5 and M5e Multi-Mode Microplate Readers User Guide Fluorescence Microplate Reader Comparison Chart. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. Shop now. ATG provides repair, maintenance, and contract services for laboratory research and bioprocess instruments. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). Bottom-reading is usually recommended for cell-based assay with adherent cells. View the Molecular Devices website. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Path Check Pathlength Measurement SpectraMax Spectrofluorometers. The absorbance was measured at 490 nm using a SpectraMax® M5 microplate reader (Molecular Devices, USA). The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. SpectraMax® M5e. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. Examples: “Press carefully to engage,” “ Do not. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. When a feature or capability applies to only certain readers, this exception is noted. Molecular Devices | Life Science Applications | Bioanalytical. For a higher . SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. 2. 3 mg. Or scan up to 384 wells at any time to determine the best assay conditions. Instrument settings are shown in Figure 3. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. The optical density (OD) was measured at 450 nm with a SpectraMax M5 microplate reader (Molecular Devices). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. For more sample throughput, the system can Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. 1. To block unwanted residual excitation light and minimize background interference, a 570 nm. Wide range of applications. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuidethe reader’s stated range. SpectraMax M Series Multi-Mode Microplate Readers 7 The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2µL samples with 24- or 64-well plates. Page 1 of 8 . degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. 40266 Email: drugdiscoverytech@lifetech. After 3 min of reaction, the fluorescence emission spectra of the mixed solutions were recorded using a multifunctional microplate reader (SpectraMax M5, Molecular Devices, USA) at the excitation wavelength (λ ex) of 485 nm. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini EM Reader, users never have to worry about not having the right set of filters. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. LMax II 384 Microplate Luminometer: Integration time = 1 second, Settling time = off, Autoread = off. 1. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The samples were boiled for 5 min with 1 ×. Taurine ≥99 % was used as standard (Sigma-Aldrich ®,. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. 0 . The advantage of using labeled probes is detection specificity. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. The absorbance was measured at 570 nm using a SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA). Five preferred detection modes. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. The Molecular Devices SpectraMax M2 Microplate Reader is a multidetection microplate reader with dualmonochromators, dual-mode cuvette ports, and top-reading capability. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. In brief, RAW 264. 1 is GxP edition only; Protocols for the ABS1 Validation Plate. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. SpectraMax® M5. M3-M5e_manual. book Page 7 Wednesday, July 7, 2010 2:09 PM. 00 USD. When the user applies a set temperature of 37°C, the top is heated to 37. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. The system. The TR-FRET signal was read on a TR-FRET microplate reader (SYNERGY H1, Biotek, Inc). Spectramax Multi-Mode Microplate Reader Performance Specifications. • SpectraMax M5 Multi-Mode Microplate Reader Figure 2. IMAP FP Settings in SoftMax Pro M5 SpectraMax M5 e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5 e models. Qualify the luminescence performance of the SpectraMax . (Shanghai, China). These lab workhorses are used in drug discovery, research, bioassay validation, and. FlexStation™ I. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. com. 67. The CellTiter-Glo assay from Promega uses luciferase. Other readers with cell imaging include PerkinElmer’s EnSight™ Multimode Plate Reader and Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader. Microplate Readers include the M3, M4, M5, and M5e models. Figure 1. Maximum signal with lowest background. Tunable wavelength selection / monochromator. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax M5 Multi-mode Plate Reader 6. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. 0 μL) instead of the sample solution was applied as control group, and. reader in the visible range between 405–750 nm with NIST and NMI. Microplate Readers Biotek Synergy H1 Hybrid Microplate. 312 gain calibration failed. Add to Cart. You can use either. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. For the Live/Dead assay, they were treated. TR-FRET assay principle. Fluorescence intensity was detected by. 96-384. 00 USD. One vial of Thaw-and-Use TIGIT Effector Cells was thawed and resuspended in cell recovery. Here the delta mP was 336, and Z’ factor was 0. Incubation. Bioz Stars score: 86/100, based on 1 PubMed citations. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuide SpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. 0 2. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. 96-1536. played in a grid format that corresponds to the wells in a microplate (all instruments) or individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). Comprehensive software and validation Comprehensive data analysis and GxP solutions SoftMax. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. Table of Contents. 59 6. used. Category 2 and 3 microplate readers use gradient heating as well. 00 USD. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. All assays were performed in at least three individual experiments, each comprising no. SoftMax Pro. SoftMax® Pro Software is the most published microplate reader control and data analysis software in the industry. SpectraMax® iD5. Next, add 10 μl of the MTT labeling reagent (5 mg/mL) to each well for 4 h. Do not touch the clear surfaces of the cuvette. 00 USD. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. The Spectr. When a. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. 6 0. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). 0 . 3 Version 7. ) by nonlinear regression using. The raw data from each individual experiment were normalized to vehicle-treated HK-2. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. Use similar settings for other Molecular Devices readers with fluorescence detection mode. SpectraMax® M5 Multi-Mode Microplate Reader (cat. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. 40266 Email: drugdiscoverytech@lifetech. Then, 150 μL of dimethyl sulfoxide was added to each well and shaken gently for 10 min. AquaMax® 4000. HTRF-compatible SpectraMax Multi-Mode Readers. Learn how you can utilize microplate readers to drive landmark discoveries. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Bookings are free, but you will need to use iLab to book. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. SpectraMax 250 User. The Perkin Elmer Wallac 1420 VICTOR2™ is a high performing microplate reader built for multi-tasking functions. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. 2 only). Turn on the microplate reader. Physical specifications and backup power UPS Choices for SpectraMax® and Gemini™ microplate readers. Maximum signal with lowest background. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. For biological applications involving small samples, such as purificationThe guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Figure 4: Transcreener ADP. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. Maximum signal with lowest background. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. M3-M5e_manual. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 0 μL) instead of the enzyme solution was used as the sample blank group. Then, after H 2 O 2 was added, the fluorescence was measured for each time period at 488 nm (excitation) and 525 nm (emission) wavelengths on a SpectraMax M5 microplate reader. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. TNF standard curve. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown menu. After that, the cells were resuspended and diluted to a suitable density using DCFH-DA working solution, transferred to an opaque 96-well plate and incubated for 30 min. Sensitive fluorescent quantitation of DNA with the Quant-iT. 1. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. The integration time suggested is 5-10 sec. 81). SpectraMax Lmicroplate luminometer. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. 4 Max StDev 1. MOLECULAR DEVICES SpectraMax M5 also includes a wide selection of curve-fitting functions, allowing users to perform accurate and reliable quantification of biochemical reactions. Table of Contents. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-,. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. The readings with excitation at 360 nm and emission at 450 nm represented the amount of. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. Add to Cart. fluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiter. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. flexibility of monochromator-based systems. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. The cells were then treated to induce apoptosis. SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. Note: this assay can also be detected using the luminescence mode of SpectraMax M5/M5 e and FlexStation 3multi-mode microplate readers. The following document is. SpectraMax® ABS. . The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . The following document is intended to demonstrate setup of this instrument. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader (Recent Model) with One Year Warranty (Svc Contract Available) Molecular Devices SpectraMax™ M5e Microplate Reader with 90 Day Depot Warranty (Svc Contract Available). Pick the best speed, sensitivity, and price for your needs. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini EM Reader, users never have to worry about not having the right set of filters. 54 1. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. 00. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. Detection cartridge modularity allows for easy addition of new read modes within minutes without. edu) to be added as a trained user in iLab. This application note describes how to. IMAP FP PDE Evaluation Kit (Molecular Devices P/N R8175)The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Molecular Devices ThermoMax Microplate Reader COMPLETE w WARRANTY $1,750. The absorbance was recorded using a SpectraMax M5 microplate reader (Molecular Devices). Microplate Readers Biotek ELx808 Microplate Reader Absorbance. A microplate reader asThe SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. 2. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM SpectraMax M5 Microplate Reader: Read type = endpoint, Read Mode = luminescence with 1000 ms integration time, Wavelengths = All, Automix = off, Auto calibration = on, Settling time = off, Autoread = off. 1** Version 7. Read one sample or up to 384 inThe SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® Plus 384 Absorbance Microplate Reader; SpectraMax® L (software version 5. Plate chamber design of the SpectraMax category 1 microplate readers. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. B: Selection of Excitation Wavelength. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. spr. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModePlate chamber design of the SpectraMax category 1 microplate readers. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Selection of Water Constant in PathCheck settings. . For the Live/Dead assay, they were treated. SpectraMax iD5 Multi-Mode Microplate Reader. Flexible for 6-well to 384-well microplates and cuvettes. All associated StakMax® stackers. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm. 2. iD3, iD5, i3, i3x, M3, M4, M5, M5e, L, and FlexStation 3 readers. The acceptance. Molecular Devices SpectraMax M2e Multi-Mode Microplate Reader w/ SoftMax Pro. Molecular Devices Spectramax M5 Microplate Reader; Related products. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates orThe optimized settings listed in Table 1 can be transferred to any plate reader of the same model, i. Italic and boldface type are used for emphasis. Linear, Orbital Slow, and Orbital Fast. However, when a specific probe is not available, or it causes off-target effects on the. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. ® reader to the computer. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email [email protected]% Triton X‐100, 10 mM potassium phosphate buffer pH 7. Five preferred detection modes. Here the delta mP was 336, and Z’ factor was 0. SpectraMax M5eは、宇宙に打ち上げられた世界初のマイクロプレートリーダーです！ NanoRacksとモレキュラーデバイスとのパートナーシップ、そしてSpaceX Commercial Resupply Services MissonでSpectraMax M5eを打ち上げた取り組みの詳細については、こちらをクリックしてください。Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. Published Dec 16, 2020 | Updated Dec 16, 2020. Both readers feature an 8-channel read head for fast reads and. This assay is based on the disappearance of hydrogen peroxide (H 2 O 2) at 240 nm in a medium containing 20 mM H 2 O 2, 0. 5 Compatible microplate readers FP Plate Reader SpectraMax® M5/M5e Multi-Mode Microplate ReaderSpectraMax M5 Multi-Mode Microplate Reader; Method. Keywords. Choose from a three- (M3), four- (M4), oron the SpectraMax Multi-Mode Microplate Readers by issuing a single. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) M5 SpectraMax M5 e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5 e models. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. Ltd. . 1. 5 87. Standard features include a. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. M5 SpectraMax M5e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5e models.